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moloney murine leukemia virus reverse transcriptase (mmlv rt) enzyme  (Promega)

 
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    Structured Review

    Promega moloney murine leukemia virus reverse transcriptase (mmlv rt) enzyme
    Moloney Murine Leukemia Virus Reverse Transcriptase (Mmlv Rt) Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mmlv+rt+enzyme/m+mlv+reverse+transcriptase/pm40368251-99-29-40
    Average 90 stars, based on 1 article reviews
    moloney murine leukemia virus reverse transcriptase (mmlv rt) enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Development of a Reverse Genetic System for Infectious Salmon Anemia Virus: Rescue of Recombinant Fluorescent Virus by Using Salmon Internal Transcribed Spacer Region 1 as a Novel Promoter
    Article Snippet: The RNA was treated with RNase-free DNase (Promega) before RT-PCR analysis. .. The primers F-UTR-S6 , F-S6, and R-EGFP ( ) were used for the reverse transcription reaction with the MMLV RT enzyme (200 U/μl; Promega) as recommended by the supplier. .. The GoTaq Green master mix (Promega) and the F-S6 or F-EGFP and R-S6 or R-EGFP primers were used for the PCR analysis.

    Article Title: STAT1 Interaction with E3-14.7K in Monocytes Affects the Efficacy of Oncolytic Adenovirus
    Article Snippet: Two micrograms of RNA was added to 1 μg of random hexamers (Promega), and the volume was made up to 14 μl with diethyl pyrocarbonate (DEPC)-treated water. .. The sample was heated at 75°C for 5 min, followed by cooling on ice for 5 min. Five microliters of Moloney murine leukemia virus (MMLV) reverse transcriptase (RT) buffer (Promega), 5 μl of 10 mM deoxynucleoside triphosphates (dNTP; Promega), and 1 μl of MMLV RT enzyme (Promega) were added and incubated at room temperature 10 min, and the mixture was then heated at 40°C for 50 min. Water (75 μl) was added to create a final volume of 100 μl, and the mixture was stored at −20°C. .. Microfluidic cards (Applied Biosystems) were used to amplify 90 immune-related genes in a single run.

    other:

    Article Title: Evaluation of cartilage, synovium and adipose tissue as cellular sources for osteochondral repair.
    Article Snippet: Osteochondral lesions are a major cause of pain and disability in several species including dogs, horses and human beings.. The objective of this study was to assess three potential sources of canine cells for their osteochondral regenerative potential.. Cartilage, synovium and adipose tissue cells were grown in pellet culture in chondrogenic or osteogenic media.

    Article Title: Co-opting of nonATP-generating glycolytic enzymes for TBSV replication.
    Article Snippet: Positive-strand RNA viruses build viral replication organelles (VROs) with the help of co-opted host factors.. The energy requirement of intensive viral replication processes is less understood.. Previous studies on tomato bushy stunt virus (TBSV) showed that tombusviruses hijack two ATP-producing glycolytic enzymes to produce ATP locally within VROs.

    Virus:

    Article Title: STAT1 Interaction with E3-14.7K in Monocytes Affects the Efficacy of Oncolytic Adenovirus
    Article Snippet: Two micrograms of RNA was added to 1 μg of random hexamers (Promega), and the volume was made up to 14 μl with diethyl pyrocarbonate (DEPC)-treated water. .. The sample was heated at 75°C for 5 min, followed by cooling on ice for 5 min. Five microliters of Moloney murine leukemia virus (MMLV) reverse transcriptase (RT) buffer (Promega), 5 μl of 10 mM deoxynucleoside triphosphates (dNTP; Promega), and 1 μl of MMLV RT enzyme (Promega) were added and incubated at room temperature 10 min, and the mixture was then heated at 40°C for 50 min. Water (75 μl) was added to create a final volume of 100 μl, and the mixture was stored at −20°C. .. Microfluidic cards (Applied Biosystems) were used to amplify 90 immune-related genes in a single run.

    Incubation:

    Article Title: STAT1 Interaction with E3-14.7K in Monocytes Affects the Efficacy of Oncolytic Adenovirus
    Article Snippet: Two micrograms of RNA was added to 1 μg of random hexamers (Promega), and the volume was made up to 14 μl with diethyl pyrocarbonate (DEPC)-treated water. .. The sample was heated at 75°C for 5 min, followed by cooling on ice for 5 min. Five microliters of Moloney murine leukemia virus (MMLV) reverse transcriptase (RT) buffer (Promega), 5 μl of 10 mM deoxynucleoside triphosphates (dNTP; Promega), and 1 μl of MMLV RT enzyme (Promega) were added and incubated at room temperature 10 min, and the mixture was then heated at 40°C for 50 min. Water (75 μl) was added to create a final volume of 100 μl, and the mixture was stored at −20°C. .. Microfluidic cards (Applied Biosystems) were used to amplify 90 immune-related genes in a single run.

    cDNA Synthesis:

    Article Title: The UPR sensor IRE1α and the adenovirus E3-19K glycoprotein sustain persistent and lytic infections
    Article Snippet: Protein bands were visualized by incubation with chemiluminescent reagent (Amersham) and the Amersham Imager 600. .. Cells were lysed in Trizol Reagent (Invitrogen) and total RNA extracted using the direct-zol RNA MicroPrep kit (Zymo Research). cDNA synthesis was carried out with MMLV RT enzyme (Promega, M170) using Oligo dT 15 mer primers (Promega, C1101). .. The amplification was done using SYBR Green JumpStart Taq ReadyMix in Applied Biosystems Quant Studio 3 Real-Time PCR System with primers listed in Supplementary Table .

    Article Title: The UPR sensor IRE1α and the adenovirus E3-19K glycoprotein sustain persistent and lytic infections.
    Article Snippet: .. Cells were lysed in Trizol Reagent (Invitrogen) and total RNA extracted using the direct-zol RNA MicroPrep kit (Zymo Research). cDNA synthesis was carried out with MMLV RT enzyme (Promega, M170) using Oligo dT 15 mer primers (Promega, C1101). .. The amplification was done using SYBR Green JumpStart Taq ReadyMix in Applied Biosystems Quant Studio 3 Real-Time PCR System with primers listed in Supplementary Table 2.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Chronic Vitamin C Deficiency Promotes Redox Imbalance in the Brain but Does Not Alter Sodium-Dependent Vitamin C Transporter 2 Expression
    Article Snippet: The purity of RNA was determined by spectrometry (Nanodrop 2000; Thermo Scientific, Wilmington, DE, USA) with absorbance ratios A260/A280 and A260/A230. .. RT-PCR was performed with 2 μg of RNA in duplicates yielding a total volume of 50 μL cDNA for each sample (MmLV RT enzyme, 5× MmLV buffer and RNasin (Promega)); 10 mM dNTPs and Oligo (dT) primers (60 μg/120 μL) (Fermentas GmbH, St Leon Roth, Germany); Random hexamer primer (2 μg/μL) (GE Healthcare, Uppsala, Sweden). ..

    Random Hexamer:

    Article Title: Chronic Vitamin C Deficiency Promotes Redox Imbalance in the Brain but Does Not Alter Sodium-Dependent Vitamin C Transporter 2 Expression
    Article Snippet: The purity of RNA was determined by spectrometry (Nanodrop 2000; Thermo Scientific, Wilmington, DE, USA) with absorbance ratios A260/A280 and A260/A230. .. RT-PCR was performed with 2 μg of RNA in duplicates yielding a total volume of 50 μL cDNA for each sample (MmLV RT enzyme, 5× MmLV buffer and RNasin (Promega)); 10 mM dNTPs and Oligo (dT) primers (60 μg/120 μL) (Fermentas GmbH, St Leon Roth, Germany); Random hexamer primer (2 μg/μL) (GE Healthcare, Uppsala, Sweden). ..



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